內(nèi)容: Kaji lemon (Citrus jambhiri) is a variety of lemon known for its high concentration of ascorbic acid, natural compounds, and cholesterol-lowering effects. However, a lack of appropriate postharvest storage can negatively affect lemon fruits’ shelf life and marketability, causing significant quality loss. Shellac wax is well-known for its film-forming, water-resistant, and gas- and moisture-barrier properties, which can be used as a coating material. In this research, shellac wax-based coating formulations were prepared by incorporating Aloe vera (AV) gel and eugenol as antioxidant and antibacterial agents to preserve the postharvest qualities of Kaji lemons during postharvest storage. The effectiveness of the coated fruits was determined by assessing various physicochemical quality parameters, including weight loss, total soluble solids, firmness, pH, acidity, and decay index, periodically at 5-day intervals during ambient storage. The obtained results revealed that the fruits treated with shellac wax containing eugenol nanoemulsion (ENE) and AV exhibited promising outcomes and prolonged shelf life of the fruits for 21 days with minimal weight loss, maintained firmness, and exhibited the least decay during ambient storage conditions. Moreover, the incorporation of eugenol oil nanoemulsion and AV gel into the coatings improved their physicochemical and functional characteristics, including enhanced antimicrobial activities against foodborne bacterial pathogens (Escherichia coli and Bacillus subtilis).

內(nèi)容: Inappropriate storage methods can shorten the shelf life of fruits, leading to fruit waste. Ethylene is an important factor affecting the shelf life of fruits. In this study, a coating with a good ethylene removal performance was prepared using a simple two-step method on Moso bamboo. The ethylene removal mechanism of the coating was evaluated using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) spectra and water contact angle (WCA) analysis. The banana preservation performance of the coating was analyzed by observing the appearance, quality, sugar content, and hardness of the bananas. This preparation method is simple, cost-effective, and convenient for large-scale production and applications. The results showed that the coating significantly extended the shelf life of bananas and reduced their visible browning. This coating reduces the loss of banana quality and minimizes the increase in sugar content and decrease in hardness during banana decay. The combination of superhydrophobicity and the preservation coating further enhanced the preservation of bananas.

內(nèi)容: Most of the Pleurotus eryngii (PE) are consumed after cooking. The aim of this study was to investigate the metabolites and flavour changes of PE under four cooking methods, namely, air frying, boiling, frying and steaming, by using metabolomics techniques, and to screen the components for network prediction of components that have the potential to prevent obesity, and to explore the underlying mechanisms. We used the UHPLC-Q-Exactive-MS/MS metabolomics method to identify and screen 35 metabolites as the major chemical components of PE, including lipids and lipid-like molecules, organic acids and derivatives, nucleosides, nucleotides, analogues, and other small molecules. In addition, a network pharmacology approach was used to identify targets such as STAT3, CASP3, and JUN were identified as playing important roles in the network. The results were validated by molecular docking. This study provides a basis for the optimization of cooking methods for PE and offers new insights for future research.

內(nèi)容: The objective of this study was to establish an efficient and low-cost method for preparing glucoraphenin (GRE) from stir-fried seeds of Raphanus sativus L., and then evaluate the anti-hyperlipidemia activity of GRE. The optimal resin was selected by comparing adsorption and desorption ability of six kinds of anion exchange resins for GRE. The loading and elution conditions of the optimal resin for GRE were then optimized by dynamic adsorption and desorption experiments. The results showed that D301-Cl- resin was the optimal resin, and the optimal loading conditions were the original sample solution (pH = 6) with a concentration of 0.10 g/mL, the sample loading flow rate was 2 BV/h, and the sample volume was 7 BV. The optimum elution conditions were as follows: the elution solution was 5 g/100 mL NaCl, followed by subsequent elution with 4 BV at an elution flow rate of 2 BV/h. The collected eluent was further purified by ultrafiltration and preparative high-performance liquid chromatography (pHPLC) to obtain GRE, and the molecular weight and structure of GRE were determined by high performance liquid chromatography time-of-flight mass spectrometry and nuclear magnetic resonance, respectively. The purity of GRE was detected to be 95.1% by HPLC. Animal experiments showed that GRE had anti-hyperlipidemic effects by improving serum and liver lipid levels, alleviating liver lipid deposition, and protecting the liver. This study indicated that GRE may be a potential anti-hyperlipidemia drug candidate or dietary supplement.

內(nèi)容: Parkinson’s disease (PD) is a progressive neurodegenerative disorder affecting movement, with no treatments currently available to halt or slow its progression. Therefore, the development of new anti-PD drugs is urgently needed. As a kind of medicine and food homologous plant, Cistanches Herba has a promising future for the treatment of PD. In this study, a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model was used to detect Cistanches Herba’s anti-PD effects via histopathology and molecular biology. Simultaneously, the effect of Cistanches Herba on the “gut microbiota-barrier axis” was assessed through gut microbiota and intestinal barrier function in mice. Finally, transcriptomics analysis was conducted to further verify the results. As a result, 37 differential metabolites and 16 microbial genera were screened and tentatively identified. Thirty-two metabolites and sixteen microbial genera were simultaneously altered with opposing trends in variation after MPTP and Cistanches Herba treatments. We built a framework for predicting targets and hostmicrobe interaction mechanisms, as well as identifying alternative treatment for PD, which should be validated further for clinical application. In conclusion, Cistanches Herba exerts a protective effect against the development of PD by manipulating the structural feature of intestinal flora to influence the host metabolites.